Evaluation of the in vitro cytogenotoxic profile of the silver(I) metal complex with furosemide (Ag-FSE)

The aim of the present study was to evaluate cytotoxic potential a new complex silver(I) with furosemide (Ag-FSE) over panel tumor and non-tumor human cells, as well its genotoxicological safety. According results obtained, cell line derived from colorectal adenocarcinoma (Caco-2) most sensitive Ag-FSE complex, among cells non-transforming fibroblasts known GM07492A, which stimulated characterization properties against these cells. In addition interfering cellular metabolic profile (evidenced by resazurin method), alters integrity membranes (results obtained Trypan blue assay) regardless evaluated. However, interference clonogenic capacity is dependent on being tested; activity evaluated survival assay evidenced Caco-2 but not GM07492A Furthermore, did induce an increase in extent DNA damage detectable comet assay, nor frequency micronuclei compared negative control, demonstrating absence genotoxicity under experimental conditions used. data provide information various biological aspects help generation drug candidates impact health area, targeting cancer treatment.